Biological properties of a newly isolated bacteriophage (NL1) that infects Escherichia coli O157:H7 strain

نویسندگان

چکیده

Escherichia coli O157: H7 strain was used as the standard for bacteriophages isolated from polluted environmental foci and animal waste-contaminated waters, some of enteroinvasive strains previously sporadic cases were to determine host diversity phages. The physiological properties specific O157:H7, designated Escherichiaphage NL1, studied. After isolation step, purified their titer increased. Cross-antigenic hosts investigated with phage NL1 phage. One-step growth curve parameters such adsorption time phages, latent period, burst size, multiplicity infection value (MOI) determined. Results showed that bacteriophage completely lysed E.coli O157:H7 (RSKK 09007) O:164 RSKK 324. In study, effects only on investigated. calculated highest determined Optimal multiplycity (2.16x108). It period lasted 20 minutes reached size at approximately 90 minutes.

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ژورنال

عنوان ژورنال: Caucasian journal of science

سال: 2022

ISSN: ['2148-6840']

DOI: https://doi.org/10.48138/cjo.1112953